Synthesis and Antimicrobial Screening of Some Novel Ferrocenyl Derivatives of Pyrazole Analogues

Pyrazoles have been studied for over a century as an important class of heterocyclic compounds and continue to attract considerable interest due to the broad range of biological activities they possess. The incorporation of the essential structural features of pyrazoles with a ferrocene moiety could provide new derivatives with unexpected and/or enhanced biological activities since several ferrocene derivatives have already been shown to be active against a number of tumors. For this reason, we investigated the synthesis of ferrocenyl-substituted pyrazoles, such as sI-alkyl/aryl-5ferrocenylpyrazoles, by employing the reaction between (2-formyl-1-chlorovinyl) ferrocene and hydrazine derivatives. Although this reaction is known, it was not studied in much detail and the low yields of ferrocenyl pyrazoles were obtained. In the determination of antimicrobial activity almost all type microbes were used in the present study were sensitive in the newly synthesised compounds and it showed a potential activity against growth of both Gram positive and Gram negative bacteria and fungus Candida albicans and Aspergillus niger. The activity was concentration dependent. Order of activity against the various microorganism used were as follows. M.aureus> C. dipththeriae> E. coil> S. typhi> B. subtilis> P.vulgaris at the diffrent concentration of 10,20 and 30 g/ well of newly synthesised compounds. It is known for the therapeutic medicinal activities against throat infections especially diphtheria. Now in our screening it was obvious that it potentially inhibited the growth of Gram positive rod C.diptheriiae and gives a scientific basis for use. It also supports its use as antiseptic in wounds. It is also note worthy that the newly synthesised compounds effectively inhibited the fungal growth of cadida albicans and Aspergilus niger at the concentration of 20g/well and showed doubled activity than that of standard in 30g/well concentration.